Protocol detail

Antibiotic Susceptibility Determination

Find Similar Protocols

For determination of zoliflodacin MICs (mg/L), agar dilution on GCVIT agar plates (Foerster et al., 2019 (link)) and microbroth dilution [in triplicates in the HFIM medium, i.e., modified Fastidious Broth (mFB)] were performed, as previously described (Jacobsson et al., 2021 (link)). Etest was used to determine MICs (mg/L) of ceftriaxone, cefixime, ciprofloxacin, and azithromycin, in accordance with the manufacturer’s instructions (bioMérieux, Marcy-l’Etoile, France).

Free full text: Click here

Jacobsson S., Golparian D., Oxelbark J., Franceschi F., Brown D., Louie A., Drusano G, & Unemo M. (2022). Pharmacodynamic Evaluation of Zoliflodacin Treatment of Neisseria gonorrhoeae Strains With Amino Acid Substitutions in the Zoliflodacin Target GyrB Using a Dynamic Hollow Fiber Infection Model. Frontiers in Pharmacology, 13, 874176.

Publication 2022

ceftriaxone cefixime

Agar Azithromycin Cefixime Ceftriaxone Ciprofloxacin Dilution Etest Mics Zoliflodacin

Top 5 similar protocols

Protocol cited in 2 other protocols

Variable analysis

independent variables

Determination of zoliflodacin MICs (mg/L)
Agar dilution on GCVIT agar plates
Microbroth dilution [in triplicates in the HFIM medium, i.e., modified Fastidious Broth (mFB)]
Etest to determine MICs (mg/L) of ceftriaxone, cefixime, ciprofloxacin, and azithromycin

dependent variables

Zoliflodacin MICs (mg/L)
Ceftriaxone MICs (mg/L)
Cefixime MICs (mg/L)
Ciprofloxacin MICs (mg/L)
Azithromycin MICs (mg/L)

control variables

GCVIT agar plates
HFIM medium (modified Fastidious Broth, mFB)
Etest methodology as per manufacturer's instructions (bioMérieux, Marcy-l'Etoile, France)

controls

Positive controls not explicitly mentioned
Negative controls not explicitly mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!