Knee joint specimens of Guinea pig were fixed and decalcified and then embedded in paraffin. The slices were then cut into 6-μm sections and mounted on slides, and the degeneration degree of tibial plateau cartilage tissue of knee joint was observed after safranin O staining and compared between groups with Osteoarthritis Research Society International (OARSI) score [12 ]. IHC staining was used for detection of Matrix Metalloproteinase-13 (MMP-13, 1:100,
PAA099Ra01,Cloud-Clone Corp.,USA) and Caspase-3 (1:200,PAA626Ra01,Cloud-Clone Corp.,USA). We quantitatively scored the IHC results accordingto the percentage of positive chondrocytes and the staining intensity, as described below. We rated the intensity of staining on a scale of 0 to 3: 0, negative; 1, weak; 2, moderate; and 3, strong. We assigned the following proportion scores: 0 if 0% of the chondrocytes showed positive staining, 1 if 0 to 1% of the chondrocytes were stained, 2 if 2 to 10% were stained, 3 if 11 to 30% were stained, 4 if 31 to 70% were stained, and 5 if 71 to 100% were stained. We then combined the proportion and intensity scores to obtain a total score (range: 0–8), as described previously [13 (link)]. The results were assessed by 2 experienced pathologists a blinded manner.
PAA099Ra01,Cloud-Clone Corp.,USA) and Caspase-3 (1:200,PAA626Ra01,Cloud-Clone Corp.,USA). We quantitatively scored the IHC results accordingto the percentage of positive chondrocytes and the staining intensity, as described below. We rated the intensity of staining on a scale of 0 to 3: 0, negative; 1, weak; 2, moderate; and 3, strong. We assigned the following proportion scores: 0 if 0% of the chondrocytes showed positive staining, 1 if 0 to 1% of the chondrocytes were stained, 2 if 2 to 10% were stained, 3 if 11 to 30% were stained, 4 if 31 to 70% were stained, and 5 if 71 to 100% were stained. We then combined the proportion and intensity scores to obtain a total score (range: 0–8), as described previously [13 (link)]
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