Standard DNA Manipulation Procedures

General DNA manipulations were performed using standard procedures. The plasmids and oligonucleotides used in this study are listed in S7 and S8 Tables, respectively. The labelling of the probes and DNA hybridization were performed according to the protocol supplied with the PCR-DIG DNA-labelling and Chemiluminescent Detection Kit (Roche). Southern and western blots experiments were performed by standard procedures [15 (link)].

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Frígols B., Quiles-Puchalt N., Mir-Sanchis I., Donderis J., Elena S.F., Buckling A., Novick R.P., Marina A, & Penadés J.R. (2015). Virus Satellites Drive Viral Evolution and Ecology. PLoS Genetics, 11(10), e1005609.

Publication 2015

PCR-DIG DNA-labelling Chemiluminescent Detection Kit

Hybridization Oligonucleotides Plasmids Western blots

Corresponding Organization : New York University

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Variable analysis

independent variables

General DNA manipulations

dependent variables

Southern and western blots experiments

control variables

Plasmids and oligonucleotides used in the study (listed in S7 and S8 Tables)
Labelling of the probes and DNA hybridization (according to the protocol supplied with the PCR-DIG DNA-labelling and Chemiluminescent Detection Kit (Roche))

controls

Positive controls
Negative controls

Annotations

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