Kupffer Cell LPS Response via Mtfmt Knockdown

Kupffer cells (BeNa Culture Collection, Kunshan, China, BNCC340733) were cultured at 37 °C in a 5% CO₂ atmosphere in 1640 medium (Wisent, cat. no.: 350-000-CL, Nanjing, China) containing 10% (v/v) fetal bovine serum. During 70% confluent growth, Kupffer cells were treated for 6 or 12 h with 100 ng/mL LPS [48 (link)]. Using jetPRIME^® transfection reagent (Polyplus Transfection, Beijing, China), specific small interfering RNAs (Mtfmt siRNA1, Mtfmt siRNA2, and Mtfmt siRNA3; GenePharma, Shanghai, China) were transfected into Kupffer cells to knock down Mtfmt. The scramble siRNA served as a negative control (NC siRNA). The sequences are listed in Supplementary Table S2.