Protein mass profiles were obtained using a MicroFlex LT MALDI-TOF Mass Spectrometer (Bruker Daltonics, Germany), with detection in the linear positive-ion mode at a laser frequency of 50 Hz within a mass range of 2–20 kDa. The setting parameters of the MALDI-TOF MS apparatus were identical to those previously used [57 (link)]. MS spectra were acquired automatically using the AutoXecute of the Flex Control v.2.4 software (Bruker Daltonics).
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