Floral Dip Transformation of A. thaliana

A. thaliana plants were transformed by a modified version of the floral dip protocol [33] (link), where only the tips of the inflorescences were dipped into the agrobacteria solution.
For selection on soil, Basta™ (glufosinate-ammonium; Bayer CropScience Deutschland GmbH, Langenfeld, Germany) was used at a concentration of 20 mg/L, both for spraying and watering. The concentrations of sulfadiazine, D-alanine, Basta™, kanamycin and hygromycin for selection on ½ MS plates were 0.75–7.5 µg/mL, 12 mM, 10 µg/mL, 50 µg/ml and 25 µg/mL, respectively.

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Lampropoulos A., Sutikovic Z., Wenzl C., Maegele I., Lohmann J.U, & Forner J. (2013). GreenGate - A Novel, Versatile, and Efficient Cloning System for Plant Transgenesis. PLoS ONE, 8(12), e83043.

Publication 2013

Agrobacteria Alanine Basta Glufosinate ammonium Hygromycin Inflorescences Kanamycin Plants Sulfadiazine

Corresponding Organization :

Other organizations : Heidelberg University

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Variable analysis

independent variables

Modified version of the floral dip protocol
Dipping only the tips of the inflorescences into the agrobacteria solution

dependent variables

Transformation efficiency of Arabidopsis thaliana plants

control variables

Concentration of Basta™ (glufosinate-ammonium) at 20 mg/L for spraying and watering
Concentrations of sulfadiazine (0.75–7.5 µg/mL), D-alanine (12 mM), Basta™ (10 µg/mL), kanamycin (50 µg/mL), and hygromycin (25 µg/mL) for selection on ½ MS plates

controls

Positive control: Not specified
Negative control: Not specified

Annotations

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