Immunoblot analysis was performed as previously described [22 (link), 23 (link)]. The following antibodies were used: anti-MGMT (1:1,000; Cell Signaling Technology, Danvers, MA, USA), anti-RAGE (1:1,000; Cell Signaling Technology, Tokyo, Japan), anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH; 1:20,000; Trevigen, Gaithersburg, MD, USA), horseradish peroxidase-linked anti-rabbit IgG (1:20,000; GE Healthcare UK Ltd., Amersham Place, Little Chalfont, UK), and horseradish peroxidase-linked whole antibody anti-mouse IgG (1:20,000; GE Healthcare).
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