Volatile Extraction from Microparticles

Volatiles from the formulated microparticles were extracted by solid-phase microextraction (SPME). A total of 0.3 g of the sample, 4.7 g of water, and 1 g of NaCl were weighed into a 10 mL glass vial. For the extraction of volatiles, SPME fiber coated with a divinylbenzene/carboxen/polydimethylsiloxane (DVB/CAR/PDMS) sorbent (50/30 µm, StableFlex™, Supelco, Bellefonte, PA, USA) was used. The method was described by Vukoja et al. [18 (link)]. Compounds were confirmed by matching their mass spectra with the National Institute of Standards and Technology mass spectral database (NIST, East Amwell Township, NJ, USA) and through their retention time (RT) and retention index (RI). Two repetitions were made for each sample. Quantification was conducted with myrtenol as an internal standard, and results were expressed as µg/kg.