Mouse Leydig and Adrenocortical Cell Culture

The MA-10 mouse Leydig tumor cell line (a gift of Dr. Mario Ascoli) was derived from the Leydig tumor, M5480P (28 (link)). The Y-1 cell line (a gift of Dr. Bernard Schimmer) was derived from a mouse adrenocortical tumor (29 (link)). General procedures for cell culture were as described previously (1 (link)). A day before the experiment, approximately 3–6 × 10⁵ cells were seeded onto the coverslip (Corning) coated with ploy-Lysine (Sigma) in wells of a six-well plate (Corning).

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Lee J, & Jefcoate C. (2017). Monitoring of Dual CRISPR/Cas9-Mediated Steroidogenic Acute Regulatory Protein Gene Deletion and Cholesterol Accumulation Using High-Resolution Fluorescence In Situ Hybridization in a Single Cell. Frontiers in Endocrinology, 8, 289.

Publication 2017

coverslip six-well plate

Adrenocortical tumor Cell culture procedures Cell line Cells Lysine Mouse Tumor Tumor cell line

Corresponding Organization : University of Wisconsin–Madison

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Variable analysis

independent variables

Cell line (MA-10 mouse Leydig tumor cell line, Y-1 cell line)

dependent variables

Not explicitly mentioned

control variables

Cell culture procedures (as described previously)
Cell seeding density (approximately 3–6 × 10^5 cells)
Cell attachment substrate (coverslip coated with poly-Lysine)

controls

Positive control: Not mentioned
Negative control: Not mentioned

Annotations

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