Agrobacterium-mediated Transient Expression

A transient expression system was developed based on Agrobacterium-mediated transformation as previously described (Zhang et al., 2011 (link)). For fluorescence observations, the tobacco leaves were excised and mounted on slides in water. Green fluorescence (GFP: 488 nm), red fluorescence (mCherry: 561 nm), and yellow fluorescence (YFP: 514 nm) were observed under a Zeiss LSM750 confocal laser-scanning microscope (Carl Zeiss AG, Oberkochen, Germany).

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Fu J, & Liu B. (2020). Exogenous Cry1Ab/c Protein Recruits Different Endogenous Proteins for Its Function in Plant Growth and Development. Frontiers in Bioengineering and Biotechnology, 8, 685.

Publication 2020

Zeiss LSM750

Agrobacterium Confocal laser scanning microscope Fluorescence Tobacco Transient

Corresponding Organization : Nanjing Institute of Environmental Sciences

Other organizations : Nanjing Agricultural University, Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences

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Variable analysis

independent variables

Agrobacterium-mediated transformation

dependent variables

Green fluorescence (GFP: 488 nm)
Red fluorescence (mCherry: 561 nm)
Yellow fluorescence (YFP: 514 nm)

control variables

Tobacco leaves
Slides
Water

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