Example 1
Three tissue samples were prepared by adding approximately 100 mg of liver tissue to a petri dish to which 250 μL Rosewell Park Memorial Institute (RPMI) medium was added. The liver tissue sample was minced to tissue pieces about 1 mm3 to about 3 mm3 in size. Tissue sample was transferred to a sample tube. The sample tube was positioned over a transducer with 4 independently operable FASA elements having a 90° angle arranged in a circular pattern having a diameter of approximately 9 mm. water bath (i.e., coupling fluid) controlled the sample temperature by setting a chiller to 25° C. The FASA elements were activated by applying RF energy to the FASA elements so that bulk lateral ultrasonic (BLU) energy is applied to the sample. Three of the four FASA elements were activated at any given time point, within the inactive FASA element rotating clockwise.
After applying the ultrasonic waves to the samples, the samples were passed through a 70 μm cell strainer, and 10 μL of the filtrate was visualized under a microscope. The high ultrasonic wave pulse frequency and a prolonged duration of the application of the ultrasonic waves resulted in cell lysis. Lower pulse frequency at a shorter duration, however, resulted in viable cells.
