Peripheral Blood Cell Profiling

The Clinical Chemistry laboratory of Karolinska University Hospital measured the absolute numbers of leukocytes, neutrophils, eosinophils, basophils, and monocytes per liter of peripheral blood using XE Sysmex flow cytometry-based analysis. Peripheral blood mononuclear cells (PBMC) were stained freshly using the following antibodies (clones): CD45RA (B56), TcRgd (B1), HLA-DR (L43), CD4 (OKT4), CD138 (ID4 or DL-101), CD19 (HIB19), NKp44 (P44-8), CD16 (3G8), CD69 (FN50), CD28 (CD28.2), CD45 (HI30), IL21R (2G1-K12), TREM-1 (TREM-26) all from Biolegend, CD3 (UCHT1) and NKG2A (Z199.1) from Beckman Coulter, IgD (IA6-2), CD14 (Mphi 9), CD27 (M-T271), CD56 (BI59) from Beckton Dickinson, NKG2D (1D11) from eBioscience. The definition of major cell populations was as described in^{35 (link)}, and performed at the Division of Rheumatology in the Center for Molecular Medicine, Karolinska Institutet.

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Hedman Å.K., Winter E., Yoosuf N., Benita Y., Berg L., Brynedal B., Folkersen L., Klareskog L., Maciejewski M., Sirota-Madi A., Spector Y., Ziemek D., Padyukov L., Shen-Orr S.S, & Jelinsky S.A. (2023). Peripheral blood cellular dynamics of rheumatoid arthritis treatment informs about efficacy of response to disease modifying drugs. Scientific Reports, 13, 10058.

Publication 2023

NKp44

Antibodies Basophils Cd138 Clinical laboratory Clones Eosinophils Flow cytometry Hla dr Il21r Leukocytes numbers Neutrophils Nkp44 Peripheral blood monocytes Peripheral blood mononuclear cells Populations Trem 1

Corresponding Organization :

Other organizations : Pfizer (United States), Karolinska University Hospital, Karolinska Institutet, Pfizer (Germany), Technion – Israel Institute of Technology

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Variable analysis

independent variables

Staining of PBMC using different antibodies (CD45RA, TcRgd, HLA-DR, CD4, CD138, CD19, NKp44, CD16, CD69, CD28, CD45, IL21R, TREM-1, CD3, NKG2A, IgD, CD14, CD27, CD56, NKG2D)

dependent variables

Absolute numbers of leukocytes, neutrophils, eosinophils, basophils, and monocytes per liter of peripheral blood

control variables

Flow cytometry-based analysis using XE Sysmex
Analysis performed at the Division of Rheumatology in the Center for Molecular Medicine, Karolinska Institutet

controls

No positive or negative controls were explicitly mentioned in the information provided.

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