Murine Melanoma Tumor Regression Study

C57BL/6 mice (7 weeks old) were weighed and then immobilized for B16-F10 murine melanoma cells inoculation (2.5 × 10⁵ cells in 100 µL of phosphate buffered saline (PBS)) subcutaneously on the dorsal flank. After 5 days, all treatments were started via intraperitoneal injection for 10 consecutive days. The experimental groups used were PBS buffer (control group); Dacarbazine (DTIC) (40 or 80 mg/kg) (SIGMA D2390); MG-Pe (50 mg/kg); and the combination of DTIC+MG-Pe (80 mg/kg + 50 mg/kg). Animals were analyzed daily by measuring tumor diameters with a digital caliper (FORD). The volume was calculated using the formula: tumor volume (cm³) = (d). (D). (D). (0.52). Being: d = smaller diameter; D = larger diameter; 0.52 = ellipse volume calculation factor [76 (link)]. At the end of treatment, animals were anesthetized with ketamine (100 mg/kg) and xylazine (10 mg/kg) and euthanized.