Immunoblotting and Immunofluorescence Assays with ARPE-19 Cells

ARPE-19 cells were planted into 6-well plates for immunoblotting, and were grown on 8-well chamber slides (Millipore, Billerica, MA, USA) for immunofluorescence. Cells were maintained in complete medium, and were harvested at 72 h post transfection. Immunoblotting and immunofluorescence were conducted using a previously described protocol^{60 (link),61 (link)}. Antibodies were detailed in Supplementary Table S3. ImageJ software (https://imagej.nih.gov/ij/index.html) was applied to determine and quantify protein expressions.

Free full text: Click here

Jiang C., Xie P., Sun R., Sun X., Liu G., Ding S., Zhu M., Yan B., Liu Q., Chen X, & Zhao C. (2018). c-Jun-mediated microRNA-302d-3p induces RPE dedifferentiation by targeting p21Waf1/Cip1. Cell Death & Disease, 9(5), 451.

Publication 2018

8-well chamber slides

Antibodies Cells Immunofluorescence Protein Transfection

Corresponding Organization :

Other organizations : Nanjing Medical University, Jiangsu Province Hospital, Zhengzhou Children's Hospital, Qilu Hospital of Shandong University, University of Sydney, Eye & ENT Hospital of Fudan University

Top 5 similar protocols

Variable analysis

independent variables

Transfection

dependent variables

Protein expression

control variables

Cell type (ARPE-19 cells)
Culture conditions (complete medium)
Time point (72 h post transfection)

controls

No positive or negative controls were explicitly mentioned in the input protocol.

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!