After enzymatic digestion, samples
were dried and alpha-Cyano-4-hydroxycinnamic acid (7 mg/mL in 50%
acetonitrile and 1% trifluoroacetic acid) spiked with a 1.67 μg/mL
[Glu]-Fibrinopeptide B internal standard was applied using an HTX
M5 Sprayer (HTX Technologies, LLC) with the following parameters:
79 °C nozzle temperature, 10 passes, 7 mg/mL concentration, 1300
velocity, 0.07 mL/min flow rate, 2.5 mm track spacing, CC pattern,
10 psi nitrogen pressure, 3 L/min gas flow rate, 0 drying, 40 mm Nozzle
height. The target plate was dried and subsequently dipped twice in
cold (4 °C) aqueous 5 mM ammonium phosphate and dried in a desiccator
until mass spectrometry data acquisition.40 (link),41 (link)
were dried and alpha-Cyano-4-hydroxycinnamic acid (7 mg/mL in 50%
acetonitrile and 1% trifluoroacetic acid) spiked with a 1.67 μg/mL
[Glu]-Fibrinopeptide B internal standard was applied using an HTX
M5 Sprayer (HTX Technologies, LLC) with the following parameters:
79 °C nozzle temperature, 10 passes, 7 mg/mL concentration, 1300
velocity, 0.07 mL/min flow rate, 2.5 mm track spacing, CC pattern,
10 psi nitrogen pressure, 3 L/min gas flow rate, 0 drying, 40 mm Nozzle
height. The target plate was dried and subsequently dipped twice in
cold (4 °C) aqueous 5 mM ammonium phosphate and dried in a desiccator
until mass spectrometry data acquisition.40 (link),41 (link)
