ARPE-19 cells (CRL-2302™) were cultured in Dulbecco’s Modified Eagle’s Medium/F-12 medium containing 10% fetal bovine serum as previously described (Park et al., 2022 (link)). Mangiferin and H2O2 (Sigma-Aldrich, St. Louis, MO, USA) were dissolved in dimethyl sulfoxide (Thermo Fisher Scientific, Waltham, MA, USA) to prepare stock solutions, diluted to appropriate concentrations in culture medium, and then treated with cells. To trigger oxidative stress, cells were cultured in medium containing H2O2, and Mangiferin and/or zinc protoporphyrin IX (ZnPP, Sigma-Aldrich) were added 1 h before H2O2 exposure.
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ARPE-19 Cell Oxidative Stress Response
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Other organizations : Dong-Eui University, Kosin University, Pusan National University, Chungbuk National University, Chung-Ang University, Jeju National University, Mokpo National University, China-US (Henan) Hormel Cancer Institute
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Variable analysis
independent variables
- Mangiferin
- Zinc protoporphyrin IX (ZnPP)
- Cellular response to oxidative stress
- ARPE-19 cells (CRL-2302™) cultured in Dulbecco's Modified Eagle's Medium/F-12 medium containing 10% fetal bovine serum
- Dimethyl sulfoxide used to dissolve mangiferin and H2O2
- Positive control: Cells treated with H2O2 to induce oxidative stress
- Negative control: Cells not treated with H2O2 or other experimental conditions
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