Hepatoma Cell Characterization Protocol

Human hepatoma cell lines,
HUH-7 and Hep3B, and human normal cell lines, HUVEC, BEAS-2B, and
QSG-7701, were obtained from Shanghai Cell Bank of Chinese Academy
of Sciences, China; RPMI-1640 culture medium, fetal bovine serum,
and trypsin were obtained from Thermo Fisher Scientific (China) Co.,
Ltd; MLs, DAPI, CK8/18/19-FITC,^{24 (link),25 (link)} CD45-PE, and magnetic
separation rack were obtained from Huzhou Lieyuan Medical Laboratory
Co., Ltd; anti-GPC3 and anti-EpCAM antibodies were obtained from Abcam;
the Prussian Blue Iron Stain Kit (Nuclear fast red) was obtained from
Beijing Solarbio Science & Technology Co., Ltd; distearoylphosphatidylethanolamine-polyethylene
glycol (DSPE-PEG), 1,2-dioleoyl-sn-glycero-3-phosphocholine
(DOPC), hexadecyl-quaternized (HQCMC), glycidyl hexadecyl dimethylammonium
chloride (GHDC), N-hydroxysuccinimide (NHS), 1-ethyl-3-(3-dimethylaminopropyl)
carbodiimide hydrochloride (EDC·HCL), and agarose were obtained
from JuKang (Shanghai) Biotechnology Co., Ltd; cholesterol, hydrochloric
acid, and other common reagents were obtained from Sinopharm Chemical
Reagent Co., Ltd; and the cell proliferation and toxicity test kit
(CCK-8) was obtained from Shanghai Yisheng Biology Co., Ltd.

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Huang Z., Li F., Zhang J., Shi X., Xu Y, & Huang X. (2022). Research on the Construction of Bispecific-Targeted Sustained-Release Drug-Delivery Microspheres and Their Function in Treatment of Hepatocellular Carcinoma. ACS Omega, 7(25), 22003-22014.

Publication 2022

HUVEC BEAS-2B RPMI-1640 culture medium fetal bovine serum trypsin hydrochloricacid

Agarose Anti antibodies Carbodiimide Cck 8 Cell Cell proliferation Chinese Cholesterol Culture medium Dapi Dopc Epcam Fetal bovine serum Fitc Gpc3 Hepatoma Human Iron N hydroxysuccinimide Normal cell lines Polyethyleneglycol Prussian blue stain Sn glycero 3 phosphocholine Toxicity test Trypsin

Corresponding Organization : Shanghai Sixth People's Hospital

Other organizations : Sun Yat-sen University, The First Affiliated Hospital, Sun Yat-sen University, Zhongshan Hospital, Fudan University, Shanghai Jiao Tong University

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Variable analysis

independent variables

Cell lines: HUH-7, Hep3B, HUVEC, BEAS-2B, QSG-7701

dependent variables

Not explicitly mentioned

control variables

Culture medium: RPMI-1640
Serum: Fetal bovine serum
Trypsin
Magnetic separation rack
Antibodies: anti-GPC3, anti-EpCAM
Stain: Prussian Blue Iron Stain Kit (Nuclear fast red)
Lipids: DSPE-PEG, DOPC, HQCMC, GHDC
Chemicals: NHS, EDC·HCL, hydrochloric acid, other common reagents
Cell proliferation and toxicity test kit: CCK-8

controls

Positive controls not explicitly mentioned
Negative controls not explicitly mentioned

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