Gluten-Free Bread Gluten Analysis

To ensure the gluten content remained below the required threshold (<3 ppm), gluten analysis was conducted on the prepared bread. To accurately measure gluten levels, specialized testing methods such as Ridascreen Gliadin (R-Biopharm AG, Darmstadt, Germany); Veratox for Gliadin R5 (Neogen, Lansing, Michigan, USA); and AgraQuant Gluten G12 (Romer Labs, Getzersdorf, Austria) were used. All values were designated as mean ± standard deviation (n = 3).
The gluten content in the gluten-free bread product was analyzed using the gliadin test Ridascreen (R-Biopharm AG, Darmstadt, Germany), which was also used by Ja Myung Yu [64 (link)].