HL-7702/SRE-Luc cells were plated in 96-well plates at a density of 2.5 × 105 cells per well and cultured for 18 hours. The cells were then switched to medium containing 5% LPDS, 10 μM compactin, and 10 μM mevalonate for 18 hours in the absence or presence of PMFE, and luciferase activity was measured as previously described (10 (link)). Normalized luciferase values were determined by dividing luciferase activity by the protein content in cell extracts quantified using the BCA (bicinchonininc acid) protein assay (Beyotime). Western blot was measured as previously described (35 (link)).
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Measuring Luciferase Activity in HL-7702/SRE-Luc Cells
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Corresponding Organization : China Pharmaceutical University
Other organizations : Zhejiang University of Technology
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Variable analysis
dependent variables
- Luciferase activity
- Protein content in cell extracts
- Cell density (2.5 × 10^5 cells per well)
- Culture duration (18 hours)
- Medium composition (5% LPDS, 10 μM compactin, 10 μM mevalonate)
- Absence of PMFE (negative control)
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