Synergistic Effects of Ceritinib and Trametinib

Twenty-five microliters of 2.5 × 10⁴ cells/ml suspension were seeded in a 384-well plate (Greiner Bio-One, Kremsmünster, Austria) and treated for 72 hours with increasing concentrations of ceritinib (Chemie Tek, Indianapolis, IN, USA) or trametinib (Chemie Tek, Indianapolis, IN, USA).^{13 (link)} Inhibition of proliferation was measured by the CellTiter-Glo Luminescent Cell Viability Assay (Promega, Madison, WI, USA). Drug synergism or antagonism was determined using Compusyn software to calculate the combination index (CI) values using the Chou-Talalay method.^{14 (link)} A CI < 1 was considered synergistic, CI > 1 was antagonistic, and CI = 1 was additive. All data represent the mean of 3 independent experiments.