Nobiletin Protects Against Cisplatin-Induced Ototoxicity

Cisplatin (P4394, Sigma-Aldrich, USA) was dissolved in saline, nobiletin (HY-N0155, MCE, USA), ferrostatin-1 (HY-100579, MCE, USA), and Z-VAD-FMK (HY-16658B, MCE, USA) were dissolved in DMSO. These solutions were diluted in culture medium. After incubation overnight, the cells and cochlear explants were treated with or without 20 µM nobiletin for 8 h, followed by exposure to 20 µM Cisplatin for 24 h. Alternatively, cells and cochlear explants were treated with 20 µM nobiletin alone for 24 h. Furthermore, based on previous study^{9 (link)}, 30 µM ferrostatin-1 and 40 µM Z-VAD-FMK were employed as inhibitors of ferroptosis and apoptosis, respectively, to pretreat cells for 2 h, followed by exposure to 20 µM Cisplatin for 24 h.