Culturing and Authenticating Multiple Myeloma Cell Lines

MM cell lines (RPMI-8226, MM.1S, KMS-11, KMS-12BM, KMS-12PE, and U-266) were obtained and cultured as described previously^{10 (link)}. Cell lines were authenticated using short tandem repeat analysis performed by JCRB (Tokyo, Japan) or BEX (Tokyo, Japan). They were also checked for mycoplasma with an EZ-PCR Mycoplasma Detection Kit (Biological Industries, Beit HaEmek, Israel) and were found to be negative. Total RNA was extracted using RNeasy Mini Kits (Qiagen, Hilden, Germany) according to the manufacturer’s instructions. EZH2 inhibitors (GSK126 and EPZ-6438) were purchased from Chemietek (Indianapolis, IN, USA). G9a inhibitors (UNC0638 and UNC0642) were from Sigma-Aldrich (St. Louis, MO, USA).