Allergic Contact Dermatitis Model

For the allergic CHS model, mice were sensitized via topical application of 0.5% (v/v) 1-Fluoro-2,4-dinitrobenzene (DNFB) (Sigma-Aldrich) in 4:1 acetone/olive oil on their shaved back (50 µL) and were challenged 4 or 5 days later with 0.2% DNFB or vehicle control (5 µL on the dorsal and 5 µL on the ventral side of the ear). Ear thickness was measured using an engineer’s micrometer (Mitutoyo, Kawasaki, Kanagawa, Japan). The mice received neutralizing IL-27 antibody (nIL-27p28AB) (R&D Systems, Minneapolis, MN), IL-15 complex (cpx), or their respective IgG control (Ctrl). To make approximately 1 µg of the IL-15 cpx, 1 µg of IL-15 (PeproTech, Rocky Hill, NJ) and 4.5 µg of IL-15 Rα (R&D Systems) were incubated for 30 minutes at 37°C (34 (link)). Each mouse received 1.2 µg of IL-15 of the cpx.