HeLa S3 cells (ATCC) were maintained in Dulbecco’s modified eagle medium (DMEM) with 10% v/v bovine calf serum (HyClone) and maintained in a 37°C, 5% CO2 environment. All media components are from Cellgro unless otherwise noted. Unless otherwise noted all reagents used in following assays are from Sigma-Aldrich. HeLa S100 cytosolic lysates were generated from cells based on the Dignam protocol as previously described.13 (link) Isolated HeLa S100 cytosolic lysates were quantified with a Nanodrop 2000 (Thermo Scientific), aliquoted, and stored at −80°C.
Protocol detail
HeLa S3 Cell Culture and Lysate Preparation
Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link:
Access Free Full Text.
Corresponding Organization : North Carolina State University
Other organizations : Louisiana State University
Top 5 similar protocols
Variable analysis
independent variables
- None explicitly mentioned
- None explicitly mentioned
- Maintenance of HeLa S3 cells in Dulbecco's modified eagle medium (DMEM) with 10% v/v bovine calf serum (HyClone) in a 37°C, 5% CO2 environment
- All media components from Cellgro unless otherwise noted
- All reagents used in following assays from Sigma-Aldrich unless otherwise noted
- Positive control: Not mentioned
- Negative control: Not mentioned
Annotations
Based on most similar protocols
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
Sign up for free or login to display all annotations
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!