Vero cells were infected with SARS-CoV-2 (MOI 0.01) for 12 h, then treated with rhein at 50 and 200 μg/mL. After 2 h of incubation, RNA was collected by TRI Reagent (T9424, Sigma-Aldrich) and quantified by NanoDrop ND-1000 UV-Vis (Thermo Scientific, Waltham, MA, USA). m6A levels were analyzed by m6A RNA methylation assay kit as already reported [49 (link)].
Free full text: Click here