The hemagglutinin (HA) epitope was detected with mouse monoclonal antibody (MAb) HA.11 (BioLegend, San Diego, CA) or rabbit polyclonal antibody (pAb) anti-HA (ThermoFisher). The Ty1 epitope was detected with mouse MAb BB2 (60 (link)). Toxoplasma-specific antibodies include mouse MAb anti-ISP1 (13 (link)), rabbit pAb anti-IMC6 (61 (link)), and mouse MAb anti-ROP7 (1B10) (62 (link)).
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