For the evaluation of the procedure, two protocols were applied to confirm the analyzer's results, [23] (ISO 9308-1:2014) and [24] (ISO 7899-2:2014). The procedure was followed by passing 100 ml of seawater through a 0,45 um Mixed Cellulose Ester (Whatman, Maidstone, UK) membrane filter via vacuum pump. After filtration, the membrane was placed on CCA and SB plates, respectively. The dishes were incubated at 37°C for 16-18 hours for CCA plates and 48 hours SB plates. Then, the confirmation of the sample for E. faecalis, continued by incubation in Bile Aesculin Agar for two hours at 44°C. The results were assessed by calculating the number of 221 bacterial colonies on plates on a colony counter (CC-J3, Bioevopeak).
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Quantifying Seawater E. faecalis
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Corresponding Organization : University of Patras
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Variable analysis
independent variables
- Protocol applied to confirm the analyzer's results
- Number of bacterial colonies on plates
- Volume of seawater passed through the membrane filter (100 ml)
- Membrane filter pore size (0.45 μm)
- Incubation temperature for CCA plates (37°C)
- Incubation duration for CCA plates (16-18 hours)
- Incubation temperature for SB plates (37°C)
- Incubation duration for SB plates (48 hours)
- Incubation temperature for Bile Aesculin Agar (44°C)
- Incubation duration for Bile Aesculin Agar (2 hours)
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