All animal
work was approved by the Institutional Animal Care and Use Committee
at North Carolina State University. Mouse MI model was generated as
previously described.45 (link),46 (link) Generally, male CD1 mice were
anesthetized with isoflurane mixed with oxygen inhalation. The heart
was exposed by a minimally invasive left thoracotomy, and LAD was
ligated permanently for induction of acute MI. After LAD ligation,
the heart was to receive one of the following four treatments randomly:
(1) MI + hCSCs in nanogel, intramyocardially injected with 1 ×
105 hCSCs in 50 μL of P(NIPAM-AA) nanogel; (2) MI
+ hCSCs in PBS, intramyocardially injected with 1 × 105 hCSCs in 50 μL of PBS; (3) MI + nanogel, intramyocardially
injected with 50 μL of P(NIPAM-AA) nanogel; (4) MI alone, MI
surgery without any injection. The hCSCs or nanogels were prelabeled
with Texas Red-X succinimidyl ester (1 mg/mL, Invitrogen) for detection.
work was approved by the Institutional Animal Care and Use Committee
at North Carolina State University. Mouse MI model was generated as
previously described.45 (link),46 (link) Generally, male CD1 mice were
anesthetized with isoflurane mixed with oxygen inhalation. The heart
was exposed by a minimally invasive left thoracotomy, and LAD was
ligated permanently for induction of acute MI. After LAD ligation,
the heart was to receive one of the following four treatments randomly:
(1) MI + hCSCs in nanogel, intramyocardially injected with 1 ×
105 hCSCs in 50 μL of P(NIPAM-AA) nanogel; (2) MI
+ hCSCs in PBS, intramyocardially injected with 1 × 105 hCSCs in 50 μL of PBS; (3) MI + nanogel, intramyocardially
injected with 50 μL of P(NIPAM-AA) nanogel; (4) MI alone, MI
surgery without any injection. The hCSCs or nanogels were prelabeled
with Texas Red-X succinimidyl ester (1 mg/mL, Invitrogen) for detection.
