Histological Assessment of Lung Injury

The middle part of tissue samples was fixed in 4% paraformaldehyde, embedded in paraffin, and cut into sections with 1‐μm thickness. Then, the sections were stained with H&E (Yuanye Biotechnology, Shanghai, China) to examine histological changes and imaged using a light microscope (Olympus, Tokyo, Japan). Two blinded pathologists participated in the assessment of the degree of lung injury. A 4‐point scale was used: none (0), mild (1), moderate (2), or severe (3). The lung injury score was obtained by summing the resulting two scores.²⁹

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Jin T., Ai F., Zhou J., Kong L., Xiong Z., Wang D., Lu R., Chen Z, & Zhang M. (2023). Emodin alleviates lung ischemia–reperfusion injury by suppressing gasdermin D‐mediated pyroptosis in rats. The Clinical Respiratory Journal, 17(3), 241-250.

Publication 2023

Embedded paraffin Light microscope Lung injury Paraformaldehyde Pathologists Tissue

Corresponding Organization : Central Hospital of Wuhan

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Variable analysis

independent variables

Fixation of tissue samples in 4% paraformaldehyde
Embedding of tissue samples in paraffin
Cutting of tissue sections with 1-μm thickness

dependent variables

Histological changes in lung tissue
Lung injury score (0-3 scale)

control variables

Staining of tissue sections with H&E
Imaging of tissue sections using a light microscope

controls

Two blinded pathologists participated in the assessment of the degree of lung injury

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