The non-targeted metabolomics method was used to analyze the metabolites in the rhizosphere soil of tea tree. This was done by taking 50 mg of soil samples and homogenizing them with 500 μL of ice-cold methanol/water (70%, v/v), respectively. The mixtures were homogenized at 30 Hz for 2 min. After homogenization, the mixture was shaken for 5 min and incubated on ice for 15 min, and centrifuged at 12,000 rpm and 4 °C for 10 min and suck 400 μL of supernatant into another centrifuge tube. 500 mL of ethyl acetate/methanol (V, 1:3) was added to the original centrifuge tube, oscillated for 5 min and incubated on ice for 15 min, centrifuged at 12,000 rpm and 4 °C for 10 min, and then took 400 mL of supernatant. The two supernatants were combined and concentrated. Then 100 μL of 70% methanol water was added into the dried product for 3min of ultrasonication. Finally, the product was centrifuged at 12,000 rpm and 4 °C for 3 min, and 60 μL of supernatant was aspirated for LC-MS/MS analysis.
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