Kidney epithelial cells MA-104 (CRL-2378.1) and Vero (CCL-81), neuronal cells SH-SY5Y (CRL-2266) and U87-MG (HTB-14) were obtained from the American Type Culture Collection Center (ATCC, USA). U87-MG, Vero, and SH-SY5Y cells were cultured in Dulbecco's modified Eagle's medium (8118305, GIBCO, USA), supplemented with 10% fetal bovine serum (FND500, ExCell Bio, Shanghai, China). MA-104 cells were cultured in modified Eagle's medium (A1451801, GIBCO, USA).
HSV-1 F strain (ATCC, USA) was grown in Vero cells and stored at − 80 °C for further use as previously described [16 (link)]. ACV-resistant strains HSV-1/153 and HSV-1/Blue were obtained from the Guangzhou Institutes of Biomedicine and Health (Guangzhou, China). HSV-1 F strain tagged by Green fluorescent protein (GFP) (GFP-HSV-1) was obtained from Professor Yuan Li of Jinan University (Guangzhou, China). HSV-2 and RV virus were obtained from the Wuhan Institute of Virology (Wuhan, China). As previously described, the influenza virus H1N1 was grown and stored at − 80 °C for further use [17 (link)]. Antibody anti-ICP0 (ab6513) and anti-gB (ab6506) were purchased from Abcam (Cambridge, UK), and anti-GAPDH (2118) was purchased from Cell Signaling Technology (Danvers, MA, USA).
HSV-1 F strain (ATCC, USA) was grown in Vero cells and stored at − 80 °C for further use as previously described [16 (link)]. ACV-resistant strains HSV-1/153 and HSV-1/Blue were obtained from the Guangzhou Institutes of Biomedicine and Health (Guangzhou, China). HSV-1 F strain tagged by Green fluorescent protein (GFP) (GFP-HSV-1) was obtained from Professor Yuan Li of Jinan University (Guangzhou, China). HSV-2 and RV virus were obtained from the Wuhan Institute of Virology (Wuhan, China). As previously described, the influenza virus H1N1 was grown and stored at − 80 °C for further use [17 (link)]. Antibody anti-ICP0 (ab6513) and anti-gB (ab6506) were purchased from Abcam (Cambridge, UK), and anti-GAPDH (2118) was purchased from Cell Signaling Technology (Danvers, MA, USA).
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