Immunofluorescence Analysis of Caveolin Proteins

The following antibodies were used: mouse anti-β-dsytroglycan (7D11; Developmental Studies Hybridoma Bank), rabbit anti-CAV3 (peptide sequence CGFEDVIAEPEGTYSFDE; Luetterforst et al., 1999 (link)), mouse anti-CAV3 (#610421; BD Biosciences), rabbit anti-Cavin4 (peptide sequence CGDDESLLLELKQSS; Lo et al., 2015 (link)), rabbit anti-Cavin4b (peptide sequence GEESEVPMYDMKQLS), mouse anti-BIN1 (clone 99D, #05-449-C; Millipore), mouse anti-actin (α-sarcomeric; clone 5C5 #A2172; Sigma-Aldrich), anti-rabbit Alexa Fluor 555 secondary antibody (#A-31572; Invitrogen), and anti-mouse Alexa Fluor 488 secondary antibody (#A-21202; Invitrogen).

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Lo H.P., Lim Y.W., Xiong Z., Martel N., Ferguson C., Ariotti N., Giacomotto J., Rae J., Floetenmeyer M., Moradi S.V., Gao Y., Tillu V.A., Xia D., Wang H., Rahnama S., Nixon S.J., Bastiani M., Day R.D., Smith K.A., Palpant N.J., Johnston W.A., Alexandrov K., Collins B.M., Hall T.E, & Parton R.G. (2021). Cavin4 interacts with Bin1 to promote T-tubule formation and stability in developing skeletal muscle. The Journal of Cell Biology, 220(12), e201905065.

Publication 2021

mouse anti-CAV3 anti-mouse Alexa Fluor 488 secondary antibody

Actin Alexa fluor 488 Alexa fluor 555 Anti antibody Antibodies Clone Hybridoma Mouse Peptide Rabbit Sarcomeric

Corresponding Organization : University of Queensland

Other organizations : Park Centre for Mental Health, Queensland Centre for Mental Health Research, ARC Centre of Excellence in Synthetic Biology, Commonwealth Scientific and Industrial Research Organisation, Queensland University of Technology, Translational Research Institute, Mater Research

Top 5 similar protocols

Variable analysis

independent variables

Mouse anti-β-dsytroglycan (7D11; Developmental Studies Hybridoma Bank)
Rabbit anti-CAV3 (peptide sequence CGFEDVIAEPEGTYSFDE; Luetterforst et al., 1999)
Mouse anti-CAV3 (#610421; BD Biosciences)
Rabbit anti-Cavin4 (peptide sequence CGDDESLLLELKQSS; Lo et al., 2015)
Rabbit anti-Cavin4b (peptide sequence GEESEVPMYDMKQLS)
Mouse anti-BIN1 (clone 99D, #05-449-C; Millipore)
Mouse anti-actin (α-sarcomeric; clone 5C5 #A2172; Sigma-Aldrich)
Anti-rabbit Alexa Fluor 555 secondary antibody (#A-31572; Invitrogen)
Anti-mouse Alexa Fluor 488 secondary antibody (#A-21202; Invitrogen)

dependent variables

Not explicitly mentioned

control variables

Not explicitly mentioned

controls

Positive controls: Not specified
Negative controls: Not specified

Annotations

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