Xenograft Model for Targeted Therapy Evaluation

Xenograft experiments received prior approval from the Institutional Animal Care and Use Committee, and were performed on n = 5–6 animals/group^{7 (link)}. In brief, 5 × 10⁶ SW620 cells were injected into either flank of male athymic nude mice (Envigo). After a week, animals were treated with vehicle or 7.5 mg/kg body weight of dBET6, thrice per week for 3 weeks by i.p. injection. Tumor volumes were measured twice per week using Vernier calipers, and the corresponding tumors were examined at the end of the study for molecular target modulation, as reported^{7 (link)}. In brief, xenografts were lysed using IP lysis buffer containing protease inhibitors^{7 (link)}, followed by immunoblotting of target proteins, as described above.