Bradykinin B1 Receptor Knockout Mice

The mice were housed in a temperature (23 ± 1°C)- and humidity-controlled facility under a 12-h dark/light cycle, fed standard mouse chow (Prolab IsoPro RMH 3000, #3005737-220, Lab Diet) and water ad libitum. The experiments were performed on adult male mice (12–16 weeks old). Bradykinin B1 receptor knockout (B1RKO) mice were a generous gift from Dr. Michael Bader (Charité Hospital, Berlin, Germany) and originated from the backcrossing of an initially mixed genetic background (129/Sc and C57Bl/6) with C57Bl/6 mice (13 (link)). Wildtype (WT) C57Bl/6NJ mice (stock no. 005304) were purchased from the Jackson Laboratory. All animal studies were approved by the East Carolina University Animal Care and Use Committee (AUP #W254) and were performed in accordance with the National Institutes of Health (NIH) Guidelines for the Care and Use of Laboratory Animals and ARRIVE guidelines.