Fluorescence Characterization of Protein Aggregation

MedChemExpress (MCE) provided the CTZ (Monmouth Junction, NJ 08852, USA). Sigma-Aldrich provided HEWL, 8-anilino-1-naphthalenesulfonic acid (ANS), Thioflavin-T (ThT), and the rest of the chemicals (St. Louis, MO 68178, USA). All experimental solutions were made to an analytical grade in double-distilled water filtered with the Milli-Q system. The pH was measured with a SENTRON INTEGRATED SENSOR TECHNOLOGY 2001 pH meter (Texas City, TX, USA). Agilent Technologies Cary Eclipse-Fluorescence Spectrophotometer was used for all fluorescence measurements (Santa Clara, USA).

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Altwaijry N., Almutairi G.S., Khan M.S., Shaik G.M, & Alokail M.S. (2023). Effect of Antihypertensive Drug (Chlorothiazide) on Fibrillation of Lysozyme: A Combined Spectroscopy, Microscopy, and Computational Study. International Journal of Molecular Sciences, 24(4), 3112.

Publication 2023

8-anilino-1-naphthalenesulfonic acid Thioflavin-T Cary Eclipse-Fluorescence Spectrophotometer

8 anilino 1 naphthalenesulfonic acid Fluorescence Thioflavin t

Corresponding Organization : King Saud University

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Variable analysis

independent variables

CTZ provided by MedChemExpress (MCE)
HEWL, 8-anilino-1-naphthalenesulfonic acid (ANS), Thioflavin-T (ThT), and the rest of the chemicals provided by Sigma-Aldrich

dependent variables

Fluorescence measurements

control variables

All experimental solutions were made to an analytical grade in double-distilled water filtered with the Milli-Q system
PH was measured with a SENTRON INTEGRATED SENSOR TECHNOLOGY 2001 pH meter
Agilent Technologies Cary Eclipse-Fluorescence Spectrophotometer was used for all fluorescence measurements

controls

No positive or negative controls were explicitly mentioned in the information provided.

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