Inflammatory Markers and Neurotransmitters in Gut

To measure inflammatory changes and neurotransmitter levels in the gut, caecum tissues were washed in sterile saline and homogenised as described previously (Dénes et al., 2010 (link)). Samples were kept at −20 °C until processing. Protein concentrations calculated using a BCA assay (Pierce/Themo Fisher Scientific). Caecum homogenates were measured for granulocyte-colony stimulating factor (G-CSF), RANTES (CCL5), KC (CXCL1), MMP-9, interleukin 6 (IL-6), ICAM-1, VCAM-1 (R & D Systems, UK), adrenaline and noradrenaline (Eagle Biosciences, NH, USA), substance P (R & D Systems, UK), and serotonin (Enzo, UK) according to the manufacturers protocol.

Free full text: Click here

Houlden A., Goldrick M., Brough D., Vizi E.S., Lénárt N., Martinecz B., Roberts I.S, & Denes A. (2016). Brain injury induces specific changes in the caecal microbiota of mice via altered autonomic activity and mucoprotein production. Brain, Behavior, and Immunity, 57, 10-20.

Publication 2016

ICAM-1 VCAM-1 substance P serotonin

Adrenaline Assay Caecum Ccl5 Cxcl1 Eagle Granulocyte colony stimulating factor Icam 1 Inflammatory Interleukin 6 Mmp 9 Neurotransmitter Noradrenaline Protein Saline Serotonin Sterile Substance p Tissues Vcam 1

Corresponding Organization : HUN-REN Institute of Experimental Medicine

Other organizations : Hungarian Academy of Sciences, Semmelweis University

Top 5 similar protocols

Variable analysis

independent variables

None explicitly mentioned.

dependent variables

Granulocyte-colony stimulating factor (G-CSF)
RANTES (CCL5)
KC (CXCL1)
MMP-9
Interleukin 6 (IL-6)
ICAM-1
VCAM-1
Adrenaline
Noradrenaline
Substance P
Serotonin

control variables

Caecum tissue samples were washed in sterile saline and homogenized as described previously (Dénes et al., 2010)
Samples were kept at −20 °C until processing
Protein concentrations were calculated using a BCA assay (Pierce/Themo Fisher Scientific)

controls

Positive controls: Not explicitly mentioned.
Negative controls: Not explicitly mentioned.

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!