Q5 High-Fidelity DNA Polymerase, Vent (exo-) DNA polymerase, Sulfolobus DNA Polymerase IV, E. coli RNA Polymerase holoenzyme, Mth RNA Ligase (as part of the 5’ DNA Adenylation kit), T4 RNA Ligase 2 truncated KQ, ET SSB, RNase H, and RNase If were purchased from New England Biolabs. TURBO DNase, SuperaseIN, SuperScript II, SuperScript III, and BSA were purchased from ThermoFisher. Streptavidin was purchased from Promega. NusA was a gift from J. Roberts (Cornell University)