Nanoparticle Characterization and Endotoxin Removal

CNP, 14 nm diameter (Carbon Black, Printex 90, Degussa, Frankfurt, Germany) were characterized and prepared as described earlier [5] (link),[7] (link). Ectoine ((S)-2-methyl-1,4,5,6-tetrahydropyrimidine-4-carboxylic acid, LPS-free, ultrapure 99%, bitop AG, Witten, Germany) and firoin mannosylglycerate, 99% bitop AG, Witten, Germany) were solubilized in PBS. Possible endotoxin contaminations were counteracted by purifying solubilized substances using endotoxin removal spin columns from Thermo Scientific (Germany).

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Autengruber A., Sydlik U., Kroker M., Hornstein T., Ale-Agha N., Stöckmann D., Bilstein A., Albrecht C., Paunel-Görgülü A., Suschek C.V., Krutmann J, & Unfried K. (2014). Signalling-Dependent Adverse Health Effects of Carbon Nanoparticles Are Prevented by the Compatible Solute Mannosylglycerate (Firoin) In Vitro and In Vivo. PLoS ONE, 9(11), e111485.

Publication 2014

Printex 90 endotoxin removal spin columns

Carbon black Carboxylic acid Ectoine Endotoxin Mannosylglycerate

Corresponding Organization : Heinrich Heine University Düsseldorf

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Variable analysis

independent variables

Carbon Black, Printex 90, Degussa, Frankfurt, Germany (CNP, 14 nm diameter)

dependent variables

Unspecified

control variables

Ectoine ((S)-2-methyl-1,4,5,6-tetrahydropyrimidine-4-carboxylic acid, LPS-free, ultrapure 99%, bitop AG, Witten, Germany)
Firoin mannosylglycerate, 99% bitop AG, Witten, Germany

controls

Positive control: Unspecified
Negative control: Unspecified

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