Cytokine and Amyloid-β Quantification

Following pentobarbital euthanasia, mice were perfused of blood as above. Brains were then harvested and deaggregated by pressing gently through 40 μm cell strainers in 3 ml of DMEM medium. The homogenate was then centrifuged (1400 × g, 5 min, 4 °C), and supernatants were collected. IL-1β, IL-6, TNF, and amyloid β protein levels were detected using standard ELISA after comparison to recombinant standard (cytokines: ab210895, ab213749, and ab212073, Abcam, Cambridge, MA; amyloid β^{64 (link)}: capture antibody: #800701, detection antibody: #805504 or #805404, 1:100, Biolegend, San Diego, CA).