Circulating TGF-β1 and CTGF in Vascular Calcification

In this study, we used cryopreserved sera from community-dwelling older adults participating in a cytokine and miRNA study for measuring circulating TGF-β1 and CTGF, based on enzyme-linked immunosorbent assays (ELISAs) (ab100647 and ab261851; Abcam). Their VC severity was determined based on a validated classification scheme for AAC [45 (link)], which also demonstrated good applicability to our patients according to our prior work [13 (link), 46 (link)]. We identified those without AAC and those with severe AAC, and compared their circulating TGF-β1 and CTGF levels using the Student’s independent t-test, in order to validate the clinical implications of TGF-β1 and CTGF in VC.

Free full text: Click here

Tsai Y.T., Yeh H.Y., Chao C.T., Huang J.W, & Chiang C.K. (2023). The context-dependent role of transforming growth factor-β/miR-378a-3p/connective tissue growth factor in vascular calcification: a translational study. Aging (Albany NY), 15(3), 830-845.

Publication 2023

ab100647 ab261851

Circulating mirna Ctgf Cytokine Enzyme linked immunosorbent assays Older adults Patients Sera Student Tgf β1

Corresponding Organization :

Other organizations : National Taiwan University Hospital, Soochow University, National Taiwan University, National Yunlin University of Science and Technology

Top 5 similar protocols

Variable analysis

independent variables

VC severity (no AAC vs. severe AAC)

dependent variables

Circulating TGF-β1 levels
Circulating CTGF levels

control variables

Community-dwelling older adults participating in a cytokine and miRNA study

controls

No positive or negative controls were explicitly mentioned.

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!