Affinity Purification of Target Proteins
Variable analysis
- Incubation of cell lysate (500 μg) or recombinant proteins (200 ng) with erianin-CNBr activated sepharose 4B
- Pull-down bands observed through Western blotting
- Incubation temperature of 4 °C
- Incubation time of overnight
- Washing the protein-beads complex four times using a rotational incubator to remove non-specific binding
- Positive control: Not explicitly mentioned
- Negative control: Not explicitly mentioned
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