Serum sIgE‐levels to honeybee venom (HBV), wasp venom (WV), hornet venom (HV), and recombinant allergens: rApi m 1, rApi m 2, icarapin (rApi m 10) from HBV, rVes v 5, phospholipase A1 (rVes v 1) from WV, and CCD were measured by Immunoblot (Euroimmun, Germany, Euroline DPA‐Dx insect venoms 3). Serum samples of 29/71 multiple‐positive patients were tested in the EUROLINE assay using the anti‐CCD Absorbent (Euroimmun). The absorbent (CCD‐inhibitor) for eliminating CCD‐sIgE was composed of glycoprotein bromelain extracted from pineapple. Serum samples were incubated with anti‐CCD Absorbent for 60 min at room temperature according to the manufacturer's instructions. The sera were retested using the same insect venoms profile, as previously mentioned.
The cut‐off value for positive sIgE‐test results was 0.35 kUA/L. The sIgE‐reactivity was categorized quantitatively into six classes: Class 1 (≥0.35 to <0.70 kUA/L), Class 2 (≥0.70 to <3.50 kUA/L), Class 3 (≥3.50 to <17.50 kUA/L), Class 4 (≥17.50 to <50.00 kUA/L), Class 5 (≥50.00 to <100.00 kUA/L), and Class 6 (≥100.00 kUA/L).
The cut‐off value for positive sIgE‐test results was 0.35 kUA/L. The sIgE‐reactivity was categorized quantitatively into six classes: Class 1 (≥0.35 to <0.70 kUA/L), Class 2 (≥0.70 to <3.50 kUA/L), Class 3 (≥3.50 to <17.50 kUA/L), Class 4 (≥17.50 to <50.00 kUA/L), Class 5 (≥50.00 to <100.00 kUA/L), and Class 6 (≥100.00 kUA/L).
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