Female Transgenic SOD1G93A Mouse Model

Female transgenic SOD1G93A mice on C57BL/6JOlaHsd (C57G93A) and 129S2/Sv (129SvG93A) genetic background, and their corresponding non-transgenic (NTG) female littermates, were used in this study [23 , 25 (link)]. The animals have been housed under SPF (specific pathogen-free) standard conditions (22 ± 1 °C, 55 ± 10% relative humidity, and 12-h light/dark schedule), 3–4 per cage, with free access to food (standard pellet, Altromin, MT, Rieper) and water. Procedures involving animals and their care were conducted in conformity with the institutional guidelines of the Mario Negri Institute for Pharmacological Research, Milan, Italy, IRFMN, which are in compliance with national (D.lgs 26/2014; authorisation no. 783/2016-PR issued on August 8, 2016, by Ministry of Health) and Mario Negri institutional regulations and policies providing internal authorisation for persons conducting animal experiments (quality management system certificate—UNI EN ISO 9001:2008—reg. N° 6121); the NIH Guide for the Care and Use of Laboratory Animals (2011 edition); and EU directives and guidelines (EEC Council Directive 2010/63/UE). Animal studies were approved by the Mario Negri Institute Animal Care and Use Committee and by the Italian Ministerial Decree no. 246/2020-PR.

Free full text: Click here

Margotta C., Fabbrizio P., Ceccanti M., Cambieri C., Ruffolo G., D’Agostino J., Trolese M.C., Cifelli P., Alfano V., Laurini C., Scaricamazza S., Ferri A., Sorarù G., Palma E., Inghilleri M., Bendotti C, & Nardo G. (2023). Immune-mediated myogenesis and acetylcholine receptor clustering promote a slow disease progression in ALS mouse models. Inflammation and Regeneration, 43, 19.

Publication 2023

Animal Female Food Genetic background Humidity Laboratory animals Light Ministerial Specific pathogen free Standard Transgenic Transgenic mice

Corresponding Organization :

Other organizations : Mario Negri Institute for Pharmacological Research, Sapienza University of Rome, Istituto Pasteur, University of L'Aquila, IRCCS Ospedale San Raffaele, Fondazione Santa Lucia, Istituti di Ricovero e Cura a Carattere Scientifico, Azienda Ospedaliera di Padova

Top 5 similar protocols

Variable analysis

independent variables

Genetic background of mice (C57BL/6JOlaHsd and 129S2/Sv)
Transgenic status of mice (transgenic SOD1G93A and non-transgenic (NTG) littermates)

dependent variables

Not explicitly mentioned

control variables

Housing conditions (22 ± 1 °C, 55 ± 10% relative humidity, 12-h light/dark schedule)
Number of mice per cage (3-4)
Access to food and water (standard pellet, free access)

controls

Positive control: Transgenic SOD1G93A mice
Negative control: Non-transgenic (NTG) female littermates

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!