After the monolayer formation, the functional amine group needs to be activated to allow the coupling of the antibody to the silanized surface.
There exist several activators commercially available that react with the amine group, such as disuccinimidyl carbonate (DSC), N-hydroxy succinimide (NHS), glutaraldehyde (GA) [29 (link),31 (link),32 (link),33 (link)]. For this purpose, we used carbonyl diimidazole CDI (115533 Sigma Aldrich) since this chemical is easy to use in water solution, and successful results have been reported in the literature [34 (link)].
Modified surfaces were immersed in a solution of 150 mM of CDI in water and stirred for 1h at room temperature. (Figure 2 B).
There exist several activators commercially available that react with the amine group, such as disuccinimidyl carbonate (DSC), N-hydroxy succinimide (NHS), glutaraldehyde (GA) [29 (link),31 (link),32 (link),33 (link)]. For this purpose, we used carbonyl diimidazole CDI (115533 Sigma Aldrich) since this chemical is easy to use in water solution, and successful results have been reported in the literature [34 (link)].
Modified surfaces were immersed in a solution of 150 mM of CDI in water and stirred for 1h at room temperature. (
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