Evaluation of Collagen Type III Expression

Immunocytochemical staining was used to evaluate the expression of collagen type III after 24 h incubation with decoction at 50 µg/mL. Betulinic acid (2 µM) was used as a positive control. The study was performed exactly as described in our previous paper [7 (link)], except that a decoction was used instead of acetone and ethanol extracts. The intensity of immunohistochemical staining was evaluated as negative (−), weak (+), moderate (++), or strong (+++), as in the previous article [7 (link),26 (link)].

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Hadzik J., Choromańska A., Karolewicz B., Matkowski A., Dominiak M., Złocińska A, & Nawrot-Hadzik I. (2023). Oral Wound Healing Potential of Polygoni Cuspidati Rhizoma et Radix Decoction—In Vitro Study. Pharmaceuticals, 16(2), 267.

Publication 2023

Acetone Betulinic acid Collagen type iii Ethanol Weak

Corresponding Organization : Wroclaw Medical University

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Variable analysis

independent variables

Decoction at 50 µg/mL

dependent variables

Expression of collagen type III

control variables

The study was performed exactly as described in the previous paper [7 (link)]

controls

Positive control: Betulinic acid (2 µM)

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