Chlorophyll a (Chl a), chlorophyll b (Chl b), total chlorophyll (Chl T), and carotenoids (CAR) were calculated using methods presented by Arnon (33) and Lichtenthaler (34) . Accordingly, 0.5 g of fresh tissue of the leaf blade for each sample was first weighed and rubbed in a stone mortar with 80% aqueous acetone. Then, the obtained scum was isolated by a filter paper and volumetric flask, and the resulting scum was again rubbed and then isolated. Next, the product was diluted to 10 ml with 80% acetone and immediately transferred to a cell and absorbed by a spectrophotometer (Jenway 6300 model) at different wavelengths of 645, 663, and 470 nm. Eventually, Chl a, b, total Chl, and CAR contents were determined using the following formulas. It should be noted that 80% aqueous acetone was used as a Blank solution. In which V is the final volume of extract per milliliter, W is tissue weight per gram, and D is optical absorption