DNA Extraction and Genotyping Protocol

DNA samples were extracted from whole blood using the salting-out method [18] (link). Genotyping was previously performed using the Illumina Metabochip (Illumina, USA) at the DNA Technologies Core of the University of California Davis (California, USA), and genotypes for the two single nucleotide polymorphisms (SNPs) of interest were extracted from data files. All details regarding the genotyping experiment and quality control of the genotypic data have been described previously [19] (link).

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Chikowore T., Sahibdeen V., Hendry L.M., Norris S.A., Goedecke J.H., Micklesfield L.K, & Lombard Z. (2019). C679X loss-of-function PCSK9 variant is associated with lower fasting glucose in black South African adolescents: Birth to Twenty Plus Cohort. Journal of Clinical & Translational Endocrinology, 16, 100186.

Publication 2019

Metabochip

Blood Genotypic Single nucleotide polymorphisms

Corresponding Organization : National Health Laboratory Service

Other organizations : South African Medical Research Council

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Variable analysis

independent variables

Single nucleotide polymorphisms (SNPs) of interest

dependent variables

Genotypes of the two single nucleotide polymorphisms (SNPs) of interest

control variables

DNA extraction method (salting-out method)
Genotyping platform (Illumina Metabochip)
Genotyping location (DNA Technologies Core of the University of California Davis)

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