Cells were fixed as previously described [47 (link)], and incubated with anti-integrin β1, anti-integrin β4, anti-paxillin, anti-E-cadherin (BD Biosciences, 610467, 611232, 6100052, and 610181, respectively), anti-integrin β3, anti-Giantin (Abcam Ab7167 and 24586 respectively), and anti-α-catenin (B52975; Calbiochem), for 1 h, and then incubated with the corresponding secondary antibodies and FITC-phalloidin (Invitrogen) at 1 μg/mL for 30 min.
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