Liver Tissue Biochemical Analysis

All experimental groups’ liver samples were homogenized in 9 mL of pH 7.4 PBS buffer after being weighed to 1 g. To obtain the supernatant, the liver homogenate was centrifuged (2500× g, 4 °C, 10 min). The activities of superoxide dismutase (SOD), malondialdehyde (MDA), glutathione (GSH), glutathione peroxidase (GSH-Px), ethanol dehydrogenase (ADH), acetaldehyde dehydrogenase (ALDH), catalase (CAT), total protein (TP), and triglyceride (TG) in liver tissue were determined according to the manufacturer’s protocol (Shandong Biobase Biodustry, Jinan, China). The levels of hepatic CYP2E1; inflammatory cytokines, including interleukin-6 (IL-6); and tumor necrosis factor-α (TNF-α) were measured by enzyme-linked immunosorbent assay (ELISA) based on the manufacturer’s instructions (Jiangsu Meimian Industrial Co., Ltd., Jiangsu, China) [33 (link),34 (link)].

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Zhang J., Fan J., Luo H., Liang Z., Guan Y., Lei X., Bo N, & Zhao M. (2023). Alleviation of Alcoholic Fatty Liver by Dendrobium officinale Flower Extracts due to Regulation of Gut Microbiota and Short-Chain Fatty Acids in Mice Exposed to Chronic Alcohol. Foods, 12(7), 1428.

Publication 2023

Acetaldehyde dehydrogenase Buffer Catalase Cyp2e1 Cytokines Dehydrogenase Enzyme linked immunosorbent assay Ethanol Glutathione peroxidase Inflammatory Interleukin 6 Liver Malondialdehyde Protein Superoxide dismutase Tissue Triglyceride Tumor necrosis factor α

Corresponding Organization : Yunnan Agricultural University

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Superoxide dismutase (SOD) activity
Malondialdehyde (MDA) level
Glutathione (GSH) level
Glutathione peroxidase (GSH-Px) activity
Ethanol dehydrogenase (ADH) activity
Acetaldehyde dehydrogenase (ALDH) activity
Catalase (CAT) activity
Total protein (TP) level
Triglyceride (TG) level
Hepatic CYP2E1 level
Interleukin-6 (IL-6) level
Tumor necrosis factor-α (TNF-α) level

control variables

PH 7.4 PBS buffer
Centrifugation conditions (2500× g, 4 °C, 10 min)

positive controls

None specified

negative controls

None specified

Annotations

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