RNA Extraction and qRT-PCR Analysis

RNA extraction and qRT-PCR methods were conducted as previously published [53 (link)]. In brief, the total RNA was extracted using Trizol (Invitrogen, Carlsbad, CA, USA), and the RNA was converted into cDNA using the ReverTra Ace qPCR RT kit (Toyobo, Osaka, Japan). Furthermore, using cDNA as a template, RT-PCR analysis was performed using the Applied Biosystems Quant Studio 3 Real-Time PCR System instrument. The primers used in this assay are listed in Supplementary Table S1.

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Guo N., Tang S., Wang J., Hu S., Tang S., Wei X., Shao G., Jiao G., Sheng Z, & Hu P. (2023). Transcriptome and Proteome Analysis Revealed That Hormone and Reactive Oxygen Species Synergetically Regulate Dormancy of Introgression Line in Rice (Oryza sativa L.). International Journal of Molecular Sciences, 24(7), 6088.

Publication 2023

Trizol ReverTra Ace qPCR RT kit Quant Studio 3 Real-Time PCR System instrument

Assay Cdna Primers Rt pcr Trizol

Corresponding Organization : China National Rice Research Institute

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Variable analysis

independent variables

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dependent variables

Gene expression levels

control variables

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controls

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